Figure 5

Modulation of 5'-phosphotyrosyl bond cleavage by Mg²⁺ in MCF-7 whole cell extract and zebrafish embryo extract. 1 μg of MCF7 whole cell extract was reacted with 5-sub (1 nM) for 10 minutes under conditions described in "Material & Methods." (A) Data obtained in Panel A was plotted (B) 2 μg and 4 μg of zebrafish embryo extract reacted with 5-sub (1 nM) (C) Data represent mean values with standard error derived from three independent experiments.