Figure 2

Over-expression and knockdown of TIMP-1 in CT-26 murine colon carcinoma cells.(A) Secretion of human TIMP-1 by stably TIMP-1-transduced cells (ELISA). (B, C) Secretion of murine TIMP-1 protein (B, ELISA) or expression of TIMP-1 mRNA (C, relative qPCR) by wild type cells or by stable clones harboring TIMP-1 shRNA or non-coding (NTC) shRNA.