IRE1 recognition sites in ZmbZIP60 mRNA and predicted protein. Diagram comparing RNA secondary structure predicted from Mfold for a segment of (A) AtbZIP60 mRNA and (B) ZmbZIP60 mRNA. (C) Partial predicted protein sequence encoded by unspliced ZmbZIP60 mRNA. Splice sites are indicated by arrows and the 20b intron is underlined. The predicted TMD is indicated in italics. (D) Partial predicted protein sequence encoded by spliced ZmbZIP60 mRNA. The arrow indicates the splice junction. The novel sequence produced by the frameshift is indicated in red. The putative nuclear localization signal is underlined. (E) Diagram showing the structures of the predicted proteins encoded by unspliced and spliced ZmbZIP60 mRNAs.