Induction of ZmbZIP60 splicing by ER stress agents. ZmbZIP60 splicing in maize seedlings was detected using a flanking primers RT-PCR assay (FP) or a specific primer assay for spliced mRNA (SPS) or unspliced mRNA (SPU). Both unspliced (slower migrating) and spliced (faster migrating) forms appear in the FP assay and the migration position of both forms is indicated by arrows. Seven-day-old seedlings were treated by (A) immersing their roots in 2.5 mM DTT solution or (B) topically spraying the seedlings with the same solution. RNA was extracted from roots and shoots. (C) Seedlings were treated with 100 μM cyclopiazonic acid (CPA) or with 5 μg/ml tunicamycin (TM). RNA was obtained from roots. Con = control (No treatment).