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Figure 1 | BMC Research Notes

Figure 1

From: An easy and versatile 2-step protocol for targeted modification and subcloning of DNA from bacterial artificial chromosomes using non-commercial plasmids

Figure 1

Schematic representation of the 2-step protocol of recombineering. (A) After induction with IPTG, BAC and pKM208 containing cells were electroporated with a CreERT2-Neo cassette with flanking homology arms. This resulted in substitution of the open reading frame by the targeting cassette using homologous recombination. (B) Cells hosting recombined BAC and pTP223 were electroporated with a pBR322-derived targeting cassette. This resulted in subcloning of a large BAC-derived DNA fragment into pBR322. Homologous regions are colored. Note, that DNA fragments are not drawn to scale.

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BMC Research Notes

ISSN: 1756-0500

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