Figure 1

Maps of mini-Tn 7 delivery vector and ori _R6Kγ reporter plasmid. A) Map of pir⁺ delivery vector pGRG36pir. Other versions include pGRG36pir-116 which contains pir-116 in place of pir⁺ and pGRG36pir-116- FKm contains a FRT-flanked Km^r marker adjacent to pir-116 as illustrated in the inset. B) Map of the ori_R6Kγ reporter plasmid pR6KT2. Abbreviations: aacC1, aminoglycoside acetyltransferase conferring gentamicin resistance; araC, encoding the AraC regulator of expression from the E. coli arabinose operon promoter P _BAD ; B1 and B2, attB1 and attB2 Gateway recombination sites; bla, β-lactamase gene conferring ampicillin resistance; FRT, Flp recombinase target site; L and R, Tn7 left and right ends; nptII, neomycin phosphotransferase gene conferring kanamycin resistance; ori_R6Kγ, R6K plasmid γ replication origin; oriT, RP4 conjugal transfer origin; pSC101ori ts, temperature-sensitive pSC101 replication origin; sacB, levansucrase-encoding gene; tnsABCD, genes encoding the Tn7 site-specific transposition pathway; uidA, glucuronidase-encoding gene.