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Table 2 Plasmids used in this study

From: A simple method for construction of pir+ Enterobacterial hosts for maintenance of R6K replicon plasmids

Plasmids

Description

Reference/Source

pGRG36a

Ampr; mini-Tn7 delivery vector with TS replicon

[11]

pGRG36GW

Ampr, Cmr; Gateway version of pGRG36

This study

pDONR221

Cmr, Kmr; Gateway cloning vector

Invitrogen

pDONR221pir

Kmr; pir+ donor vector

This study

pDONR221pir-116

Kmr; pir-116 donor vector

This study

pGRG36pir

Ampr; pir+ delivery vector

This study

pGRG36pir-116

Ampr; pir-116 delivery vector

This study

pGRG36pir-116-FKm

Ampr, Kmr; pir-116-FKm delivery vector

This study

pFKM4

Ampr, Kmr; pFKM2 [26] with Xba I and Spe I sites removed and Pac I site introduced

This study

pR6KT2

Gmr; Sucs; oriR6Kγ reporter plasmid

This study

  1. aThe DNA sequence of pGRG36 can be obtained from GenBank under accession number DQ460223. Sequences of other plasmids constructed in this study are available from the authors upon request.