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Figure 2 | BMC Research Notes

Figure 2

From: Effects of 2 weeks lower limb immobilization and two separate rehabilitation regimens on gastrocnemius muscle protein turnover signaling and normalization genes

Figure 2

Data for Study 1. A: mRNA expression measured by RT-qPCR. mRNA data are normalized to geometric means of Cyclophillin A, β2-microglobulin and RPLP0 and are relative to individual PRE values. Target genes are presented in the left side panel, whereas putative housekeeping genes are presented in the right side panel. Data are presented as back-transformed mean ± SEM. Light gray columns represent IMMO and dark gray REHAB. B: Total protein and protein phosphorylation measured by Western blot, relative to individual PRE values. Data are presented as back-transformed mean ± SEM. Total Akt is represented twice as it was measured along with each phospho-Akt. Light gray columns represent IMMO and dark gray REHAB. * denotes a difference from PRE with 0.05 > p ≥ 0.01. ** denotes a difference from PRE with 0.01 > p ≥ 0.001. Underscored asterisks denote main (time) effects.

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