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Table 1 Natural transformation of environmental isolates of bacteriaa

From: An investigation of horizontal transfer of feed introduced DNA to the aerobic microbiota of the gastrointestinal tract of rats

Transformable isolateb Phylogenetic position Plasmids tested 16S-rRNA gene identityc
Marinobacter sp. Gammaproteobacteria; pM1, pM2, 77
  Alteromonadales pM3  
Kocuria rosea Actinobacteria; pM2 79
Photobacterium Gammaproteobacteria; pM1, pM2, 86
phosphoreum Vibrionales pM3  
Psychrobacter Gammaproteobacteria; pM1, pM2, 96
marincola Pseudomonadales pM3  
  1. aOutcome of a study examining approximately 30 isolates obtained from three sources (Mediterranean, Celtic and north Atlantic seas).
  2. bTransformants were species identified by 16S sequencing and confirmed by selective plating and PCR. The screen for transformability did not aim to be quantitative, however, the overall CFU numbers indicated a transformation frequency of ~ 10-5 to 10-6 transformants per total CFU. The utility of the plasmids for transformation studies was analyzed by use of marine isolates since the recombinant plasmids for genomic integration carried rRNA genes from a marine Psychrobacter isolate.
  3. cPercentage identity to 16S rRNA of Psychrobacter.