|
Transformable isolateb
|
Phylogenetic position
|
Plasmids tested
|
16S-rRNA gene identityc
|
|---|
|
Marinobacter sp.
|
Gammaproteobacteria;
|
pM1, pM2,
|
77
|
| |
Alteromonadales
|
pM3
| |
|
Kocuria rosea
|
Actinobacteria;
|
pM2
|
79
|
|
Photobacterium
|
Gammaproteobacteria;
|
pM1, pM2,
|
86
|
|
phosphoreum
|
Vibrionales
|
pM3
| |
|
Psychrobacter
|
Gammaproteobacteria;
|
pM1, pM2,
|
96
|
|
marincola
|
Pseudomonadales
|
pM3
| |
- aOutcome of a study examining approximately 30 isolates obtained from three sources (Mediterranean, Celtic and north Atlantic seas).
- bTransformants were species identified by 16S sequencing and confirmed by selective plating and PCR. The screen for transformability did not aim to be quantitative, however, the overall CFU numbers indicated a transformation frequency of ~ 10-5 to 10-6 transformants per total CFU. The utility of the plasmids for transformation studies was analyzed by use of marine isolates since the recombinant plasmids for genomic integration carried rRNA genes from a marine Psychrobacter isolate.
- cPercentage identity to 16S rRNA of Psychrobacter.
