Transformable isolateb | Phylogenetic position | Plasmids tested | 16S-rRNA gene identityc |
---|
Marinobacter sp. | Gammaproteobacteria; | pM1, pM2, | 77 |
 |
Alteromonadales
| pM3 | Â |
Kocuria rosea
| Actinobacteria; | pM2 | 79 |
Photobacterium
| Gammaproteobacteria; | pM1, pM2, | 86 |
phosphoreum
|
Vibrionales
| pM3 | Â |
Psychrobacter
| Gammaproteobacteria; | pM1, pM2, | 96 |
marincola
|
Pseudomonadales
| pM3 | Â |
- aOutcome of a study examining approximately 30 isolates obtained from three sources (Mediterranean, Celtic and north Atlantic seas).
- bTransformants were species identified by 16S sequencing and confirmed by selective plating and PCR. The screen for transformability did not aim to be quantitative, however, the overall CFU numbers indicated a transformation frequency of ~ 10-5 to 10-6 transformants per total CFU. The utility of the plasmids for transformation studies was analyzed by use of marine isolates since the recombinant plasmids for genomic integration carried rRNA genes from a marine Psychrobacter isolate.
- cPercentage identity to 16S rRNA of Psychrobacter.