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Table 5 PCR cycling conditions

From: An investigation of horizontal transfer of feed introduced DNA to the aerobic microbiota of the gastrointestinal tract of rats

Primers Initial Denaturation Denaturation Annealing Elongation Cycles Terminal Elongation
KanR F and ITSR-Eub R 95°C/5 min 95°C/30 s 54°C/25 s 72°C/70 s 35 72°C/7 min
16S-926 F and GFP R: 95°C/5 min 95°C/30 s 53°C/40 s 72°C/90 s 35 72°C/7 min
16S-Pr.mir-530 F and GFP F 95°C/5 min 95°C/30 s 63°C/25 s 72°C/70 s 35 72°C/7 min
16S 94°C/15 min 94°C/4 min 50°C/45 s 72°C/1 min 5  
16S (cont.)   92°C/45 s 55°C/45 s 72°C/1 min 30 72°C/7 min
13 F-14 F 97°C/5 min 97°C/30 s 50°C/45 s 72°C/1.5 min 30 72°C/7 min
16 F-17 F 97°C/5 min 97°C/30 s 50°C/45 s 72°C/1 min 30 72°C/7 min
E9F-K1 97°C/5 min 97°C/30 s 56°C/45 s 72°C/1.5 min 30 72°C/7 min
Kmup-97ar 97°C/5 min 97°C/30 s 56°C/45 s 72°C/2.5 min 30 72°C/7 min