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Figure 2 | BMC Research Notes

Figure 2

From: Establishment of a PCR analysis method for canine BRCA2

Figure 2

Identification of splicing variants within exon 11 in normal testes. The splicing variants identified in normal testes lacked a large portion of exon 11. (A) To confirm the presence of a novel BRCA2 transcript, splicing variants of the cDNA from total RNA of the testes were amplified using nested PCR. (B-E) Electropherogram showing the direct sequencing data and overview of the novel BRCA2 transcript that lacked nucleotides 2390 to 6429 (B), 2390 to 6429 and 6588 to 6908 (C), 2390 to 3380, and 4649 to 6429 (D) and 2085 to 2321 and 2390 to 6429 (E) from Testis-1, -3, -4, and -5, respectively. The Testis-1 was the same sample used to generate the data in Figure 1C. Primer sets for each lane are shown in Table 1. The "M" indicates the molecular size marker (1-kbp DNA ladder). Arrowheads indicate novel BRCA2 transcripts.

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