Establishment of a PCR analysis method for canine BRCA2

BMC Research Notes

Table 2 Comparison between our sequences from the canine BRCA2 open reading frame with registered sequences

Nucletide location^a		516 T > C	1366 T > G	2428 T > G	2609A > C	4481A > C	7126delGTT	8257 T > C	10204insAAA
Amino acid		I103T	C386W	Silent	K801Q	T1425P	2307delL	Silent	M3332IK
Coding exon		3	10	11	11	11	12	18	27
Novel or reported variation		Novel	Reported	Novel	Reported	Reported	Reported	Novel	Reported
Present resequencing results^b
Full length	Ovary 1	T/C	G/G	T/G	C/A	A/C	ins/del	T/C	ins/ins
	Ovary 2	T/T	G/G	T/T	C/C	A/A	del/del	T/T	ins/ins
	Testis	T/T	G/G	T/T	C/C	A/A	del/del	T/T	ins/ins
Partial	Genome	N. D.	T:G =	N. D.	A:C =	N. D.	ins:del =	N. D.	del:ins =
			12:30		29:15		25:15		17:29
Registered sequences^c
Ochiai et al.	Testis	T	T	T	A	A	ins	T	del
Genome project	Genome	T	T	T	A	A	ins	T	del
Bignell et al.	Genome	-	-	T	A	A	-	-	-
Hsu et al.	Mammary gl.	-	-	T	A	A	-	-	-
Total allele frequency		T:C =	T:G =	T:G =	A:C =	A:C =	ins:del =	T:C =	del:ins =
		7:1	14:36	12:1	37:20	12:1	28:20	7:1	19:35
Consensus sequence		516 T	1366 G	2428 T	2609A	4481A	7126insGTT	8257 T	10204insAAA
		(103I)	(386 W)	(740 G)	(801 K)	(1425 T)	(2307insL)	(2638I)	(3332IK)

N. D., not determined.
^aNucleotide and amino acid location is based on AB043895.5
^bFull-length sequence was determined by cDNA sequencing (Accession number; AB622997, AB622998 and AB622999). When frequencies of major and minor alleles were nearly equal or were inconsistent with reported sequences, alleles were further analyzed by partial sequencing of blood genome DNA from 20-23 dogs (Table 3 and 4).
^cSequence from one dog was regarded as one allele because allele type analyses have not been described in these reports. The study by Hsu et al. examined three dogs, while others studied only one dog. Accession numbers for sequences reported by Ochiai et al., the Genome Project, Bignell et al. and Hsu et al. are AB043895.5, NC_006607.2, Z75664 and FJ464397-FJ464400, respectively.

ISSN: 1756-0500