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Figure 4 | BMC Research Notes

Figure 4

From: DNA fragments binding CTCF in vitro and in vivo are capable of blocking enhancer activity

Figure 4

Relative DNA content of three constructs: pPNT/E-CTCF7-mP that contains the CTCF7 fragment between the promoter and enhancer, pPNT/EmpS-CTCF7 that contains the CTCF7 fragment outside the promoter-enhancer pair, and pPNT/E-l-mp--the control construct with a lambda DNA fragment inserted between the promoter and enhancer in genomic DNA of CHO cells after positive-negative selection. The relative content was estimated by real-time PCR based on the difference between the number of PCR cycles required to detect the target (for detail, see Methods).

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