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Table 2 Primer pairs for identification of CTCF-binding sequences in direct and reverse orientation

From: DNA fragments binding CTCF in vitro and in vivo are capable of blocking enhancer activity

Fragment ID Combination of primers*
  Direct orientation Reverse orientation
  pPNT/EmP pPNT/EmPS pPNT/EmP pPNT/EmPS
1 P1R, 1L (522)   P1R, 1R (286)  
2 P1R, 2L (268)   P1L, 2L (290)  
3 P1R, 3L (233) P2R, 1L (319) P1L, 3L (255)  
4 P1R, 4L (346)   P1R, 4R (317)  
5 P1R, 5L (307)   P1L, 5L (329)  
6 P1R, 6L (447)   P1R, 6R (320)  
7 P1R, 7L (292) P2R, 7L (378) P1R, 7R (305)  
8 P1R, 8L (341)   P1R, 8R (330) P2R, 8L (416)
9 P1L, 9R (267)   P1R, 9R (245)  
10 P1R, 10L(557)   P1L, 10L (579)  
sns P1R, snsL (354)   P1R, snsR (368)  
  1. *Expected PCR product lengths (bp) are indicated in parentheses