Table 2 Primer pairs for identification of CTCF-binding sequences in direct and reverse orientation
From: DNA fragments binding CTCF in vitro and in vivo are capable of blocking enhancer activity
Fragment ID | Combination of primers* | |||
|---|---|---|---|---|
| Â | Direct orientation | Reverse orientation | ||
| Â | pPNT/EmP | pPNT/EmPS | pPNT/EmP | pPNT/EmPS |
1 | P1R, 1L (522) | Â | P1R, 1R (286) | Â |
2 | P1R, 2L (268) | Â | P1L, 2L (290) | Â |
3 | P1R, 3L (233) | P2R, 1L (319) | P1L, 3L (255) | Â |
4 | P1R, 4L (346) | Â | P1R, 4R (317) | Â |
5 | P1R, 5L (307) | Â | P1L, 5L (329) | Â |
6 | P1R, 6L (447) | Â | P1R, 6R (320) | Â |
7 | P1R, 7L (292) | P2R, 7L (378) | P1R, 7R (305) | Â |
8 | P1R, 8L (341) | Â | P1R, 8R (330) | P2R, 8L (416) |
9 | P1L, 9R (267) | Â | P1R, 9R (245) | Â |
10 | P1R, 10L(557) | Â | P1L, 10L (579) | Â |
sns | P1R, snsL (354) | Â | P1R, snsR (368) | Â |