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Figure 3 | BMC Research Notes

Figure 3

From: Validation of a set of reference genes to study response to herbicide stress in grasses

Figure 3

Determination of the optimal number of reference genes for accurate normalisation. geNorm calculates pairwise variations (V n/n+1 ) between consecutively ranked normalisation factors NF n and NF n+1 (NF i is the geometric mean of the expression values for the i first-ranked candidate reference genes). Each pairwise variation value is compared to the cutoff value (0.15, horizontal dotted line). For example, V2/3 corresponds to the pairwise variation between the normalisation factors of the two first-ranked (2) and the three first-ranked (3) reference genes.

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