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Table 3 Ranking of the candidate reference genes according to their stability value using BestKeeper and NormFinder

From: Validation of a set of reference genes to study response to herbicide stress in grasses

  BestKeeper    NormFinder
Reference genesa All samples   All samples Herbicide effect Phenotype effect
        BEFORE/+2.5H+6H SENSITIVE/RESISTANT
  SDb r c Ranking order   SVd Ranking order SV Ranking order SV Ranking order
26S 0.46 0.80* 1   0.335 1 0.148 1 0.132 1
TUB 1.07 0.88* 2   0.488 2 0.204 4 0.156 2
GAPDH 0.93 0.70* 3   0.494 3 0.188 3 0.242 4
UBQ 0.81 0.67 4   0.515 4 0.175 2 0.254 5
25S 0.50 0.53 5   0.669 5 0.258 5 0.279 6
18S 0.96 0.42 6   1.039 7 0.378 7 0.370 7
EF1 1.45 0.85* 7   0.813 6 0.332 6 0.231 3
  1. aThe three reference genes selected are in bold
  2. bStandard deviation of Cq values. SD values higher than the cut-off value (1.00) are underlined
  3. cPearson coefficient of correlation
  4. dStability value
  5. *: associated p value <0.001