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Table 3 Ranking of the candidate reference genes according to their stability value using BestKeeper and NormFinder

From: Validation of a set of reference genes to study response to herbicide stress in grasses

 

BestKeeper

  

NormFinder

Reference genesa

All samples

 

All samples

Herbicide effect

Phenotype effect

       

BEFORE/+2.5H+6H

SENSITIVE/RESISTANT

 

SDb

r c

Ranking order

 

SVd

Ranking order

SV

Ranking order

SV

Ranking order

26S

0.46

0.80*

1

 

0.335

1

0.148

1

0.132

1

TUB

1.07

0.88*

2

 

0.488

2

0.204

4

0.156

2

GAPDH

0.93

0.70*

3

 

0.494

3

0.188

3

0.242

4

UBQ

0.81

0.67

4

 

0.515

4

0.175

2

0.254

5

25S

0.50

0.53

5

 

0.669

5

0.258

5

0.279

6

18S

0.96

0.42

6

 

1.039

7

0.378

7

0.370

7

EF1

1.45

0.85*

7

 

0.813

6

0.332

6

0.231

3

  1. aThe three reference genes selected are in bold
  2. bStandard deviation of Cq values. SD values higher than the cut-off value (1.00) are underlined
  3. cPearson coefficient of correlation
  4. dStability value
  5. *: associated p value <0.001
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BMC Research Notes

ISSN: 1756-0500

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