Figure 2

Microscopic images of RPE cultures. A: Expansion of spheroid colony from young cultures (passage 3). B: Free colony floating in supernatant (passage 3). C, D: Re-cultured free colonies and establishment of a new RPE monolayer (passage 5). E, F, G: Ordered arrangement of RPE cells cultured on AF-coated surfaces (passage 9) H, I, J: Chaotic spread of RPE cells grown on FBS-coated surfaces (passage 9). Magnifications: 200.