Figure 3

Fluorescence microscopy of RPE cells. A, B: Fluorescence microscopy of RPE cells from the first culture, indicating an RPE cell culture purity of nearly 100%. immunostaining procedure was carried out as described in the methods. A: Cytoplasmic and granular expression of RPE65 in cells positive for RPE65 after immunostaining with rabbit anti-human RPE65 polyclonal antibody, B: Cytoskeletal expression of cytokeratin 8/18 in RPE cells positive for cytokeratin 8/18, immunostained with mouse anti-human cytokeratin 8/18 antibody. C: Decrease in cytokeratin 8/18 expression at passage 7 of RPE cultures. D-G: Negative control for cytokeratin 8/18 (E) and RPE65 (G) expression, showing no non-specific binding of the aforesaid markers. As detailed in the methods section, the negative controls were not stained with any primary antibodies. Nuclei were counter-stained with DAPI. Magnification: 200.