Enhanced generation of retinal progenitor cells from human retinal pigment epithelial cells induced by amniotic fluid

Table 1 Medium used for cases and controls

Treatment/control cases	10% AF case	10% AF control	20% AF case	20% AF control	30% AF case	30% AF control	10% FBS case	10% FBS control
Supplemented medium for first 24 hours	10% AF	10% AF	20% AF	20% AF	30% AF	30% AF	10% FBS	10% FBS
Supplemented medium for subsequent hours	10% AF	DMEM/F12 without AF	20% AF	DMEM/F12 without AF	30% AF	DMEM/F12 without AF	10% FBS	DMEM/F12 without FBS

Medium used for AF and FBS treatment and their controls is mentioned in the table above. This condition was continued for immunocytochemistry, ELISA immunoassay and RNA extraction. Each experiment has been explained in the methods.

ISSN: 1756-0500