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Table 2 Amplicon length and sequences of sense and anti-sense primers

From: Enhanced generation of retinal progenitor cells from human retinal pigment epithelial cells induced by amniotic fluid

Sequence definition

Product length

Sense primer

Anti-sense primer

VSX-1

103

AGACTCCGTGCTCAACTC

TCCTGGCTTCCTTATCATCC

CHX10

135

TCGTGATATGCTGCTTGTG

CTGTGGCTTCGTAGATGTC

PAX6

120

TTGCTGGAGGATGATGAC

CTATGCTGATTGGTGATGG

GAPDH

77

ACAGTCAGCCGCATCTTC

CTCCGACCTTCACCTTCC

  1. Primers for PAX6, CHX10, VSX-1 and GAPDH were optimized for the SYBR green assay using Beacon Designer software 7.0 (http://www.primebiosoft.com).
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BMC Research Notes

ISSN: 1756-0500

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