Figure 5

Effects of the fractionated SRL hot-water extract samples on cell proliferation and mRNA expression. (A) Anti-proliferative effects of SRL extract samples fractionated according to the polarity of constituents on the growth of MTS-C H7 cells after 24-h treatment. MTS-C H7 cells were treated with mouse IL-1β (10 ng/mL); SRL (50 μg dry matter/mL); hexane, diethyl ether, ethyl acetate, and n-butanol extract samples (all 50 μg dry matter/mL); or residual water sample (50 μg dry matter/mL). Cell proliferation was assessed by the WST-1 assay. Results are expressed as mean (SD), n = 3. Significantly different from IL-1β: * p < 0.05. The experiment was performed in triplicate. (B) MTS-C H7 cells were treated with mouse IL-1β (10 ng/mL), SRL (50 μg dry matter/mL), n-butanol extract sample (50 μg dry matter/mL), or residual water sample (50 μg dry matter/mL). Gene expression was detected by RT-PCR. Results are expressed as mean (SD), n = 3. Significantly different from IL-1β: * p < 0.05. The experiment was performed in triplicate.