Figure 2

Leukocytes survive exposure to highly leukotoxic A. actinomycetemcomitans in the presence of Guava. Viability (propidium iodide-(PI) uptake) of human mononuclear leukocytes exposed to A. actinomycetemcomitans from the highly leukotoxic strain HK1519 in a ratio of 10 bacteria/leukocyte for 60 min in presence or absence of extracts (0.1%) from different chewing stick materials. The proportion of PI-stained dead cells was quantified by flow cytometry analyses. Mean ± SD of 3 experiments is shown and significant leukotoxin neutralization is indicated (*).