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Table 2 Mouse PAb reactions against DENV-2 NS1 glycoprotein peptides prepared in the natural and reverse orientations

From: Epitope reactions can be gauged by relative antibody discriminating specificity (RADS) values supported by deletion, substitution and cysteine bridge formation analyses: potential uses in pathogenesis studies

Peptide/Epitopea N/Rb Sequencec Average ELISA absorbance (492 nm) of PAbse
    C57BL or B10 mouse haplotype anti-DENV-2 NS1glycoprotein PAbsd
C57 S RIII G BR D2N A S(C)
N-TERM + 5-VVSWKNKELK C-15 0.87 0.92 0.78 0.71 0.67 0.70 0.68 0.18
N-TERM - 15-CKLEK NKWSVV-5 0.56 0.58 0.54 0.56 0.43 0.51 0.55 0.14
LD2 + 25-VHTWTEQYK-33 0.67 0.54 1.21 1.32 0.69 0.72 1.02 0.27
LD2 - 33-KYQETWTHV-25 0.44 0.37 0.83 1.24 0.26 0.45 0.77 0.25
24A + 61-TRLE NLMWK-69 0.80 0.77 0.79 0.56 0.43 0.45 0.46 0.28
24A - 69-KWMLNELR T-61 0.35 0.32 0.52 0.42 0.41 0.28 0.40 0.25
N'LX1 + 109-TELR YSWKT-117 1.23 1.41 1.01 0.97 1.14 1.16 0.96 0.27
N'LX1 - 117-TKWSYRLE T-109 0.98 0.86 0.82 0.88 0.99 1.06 0.89 0.22
LX1 + 113-YSWKTWGKA-121 0.87 1.08 0.44 0.23 0.30 1.16 0.21 0.13
LX1 - 121-AKGWTKWSY-113 0.28 0.39 0.63 0.42 0.34 0.34 0.36 0.18
LX2/1 + 209-TWKIEK ASF-217 0.72 0.68 1.04 1.01 0.72 0.50 0.68 0.20
LX2/1 - 217-FSAKEIK WT-209 0.81 1.20 1.15 1.03 1.14 0.65 0.61 0.24
LX2/2 + 267-PWHLGKLE M-275 0.92 0.79 1.02 1.04 0.76 0.68 0.63 0.21
LX2/2 - 275-MELK GLHWP-267 0.98 1.03 1.06 1.12 0.82 0.54 0.67 0.18
24C + 301-TTASGKLIT-309 0.65 0.74 0.61 0.66 0.47 0.40 0.43 0.13
24C - 309-TILKGSATT-301 0.41 0.47 0.51 0.56 0.42 0.39 0.48 0.12
LX2/3 + 331-YGMEIR PLK-339 0.65 0.71 1.24 1.01 0.69 0.81 0.62 0.26
LX2/3 - 339-KLPRIE MGY-331 0.61 1.11 1.21 1.16 0.51 0.66 0.59 0.20
  1. aName of the epitope or MAb 1G5.4-A1-C3 and/or 1C6.3 defined N’LX1, LX2/1, LX2/2 and LX2/3 peptide sequences that contained ELK/KLE-type or KELK/KLEK-type motifs.
  2. bSynthetic peptide sequences prepared in either the natural (N: +) or reverse (R: -) orientations.
  3. cSequence position and amino acid sequence of the DENV-2 NS1 glycoprotein represented by synthetic peptides prepared both the natural and reverse orientations. The ELK/KLE-type and KELK/KLEK-type motifs are underlined.
  4. dGroups of C57BL (C57) (H-2b) or congeneic (H-2) B10 mouse strains (B10.S (S), B10.RIII (RIII), B10.BR (BR), B10.D2N (D2N) and B10.A (A)) immunized with the native hexameric DENV-2 e/sNS1 glycoprotein (gp), or the control protein (ovalbumin) (B10.S(C): (S(C)).
  5. eAverage ELISA absorbance values of mouse PAb pools reacted with duplicate sets of synthetic peptides (standard deviation range: 0.00 (low values) to 0.04 (high values)). PAb absorbance (Abs) values which were ≥ 0.650 against the peptide prepared in the natural orientation that showed ≥ 1.50-fold or ≥ 2.50-fold RADS values (Abs against the natural (+)/reverse orientated peptide sequence), are underlined and shown in bold, respectively.