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Table 3 Relative antibody discriminating specificity (RADS) values of MAbs and PAbs against the DENV-2 NS1 glycoprotein peptides

From: Epitope reactions can be gauged by relative antibody discriminating specificity (RADS) values supported by deletion, substitution and cysteine bridge formation analyses: potential uses in pathogenesis studies

MAb/PAba Relative antibody discriminating specificity (RADS) valuec
Peptide/epitope on DENV-2 NS1 glycoprotein and sequence positionb
N-TM LD2 24A N'LX1 LX1 LX2/1 LX2/2 24 C LX2/3
5-15 25-33 61-69 109-117 113-121 209-217 267-275 301-309 331-339
MAb          
1H7.4 * 16.8 * * * * * * *
5H4.3 * * 4.2 * * * * * *
3D1.4 * * * * 15.0 * * * *
1G5.3 * * * * * * * 12.4 *
1C6.3 1.12 1.05 0.47 0.93 * 0.82 1.12 * 1.67
1G5.4-A1-C3 1.10 0.88 0.62 1.26 * * * * 0.97
PAb          
C57BL 1.55 1.52 2.28 1.26 3.11 0.89 0.94 1.59 1.07
B10.S 1.59 * 2.40 1.64 2.77 0.57 0.77 1.58 0.64
B10.RIII 1.44 1.46 1.52 1.24 * 0.90 0.96 * 1.02
B10.G 1.27 1.07 * 1.10 * 0.98 0.93 1.18 0.87
B10.BR 1.56 2.65 * 1.15 * 0.63 0.93 * 1.35
B10.D2N 1.37 1.33 * 1.09 * * 1.26 * 1.23
B10.A 1.24 1.33 * * 1.08 1.11 * * *
  1. aMonoclonal antibody (MAb) name or polyclonal antibody (PAb) pools from congeneic mouse strains (C57BL, B10.S, B10.RIII, B10.BR, B10.D2N and B10.A) generated against the DENV-2 NS1 glycoprotein.
  2. bName of epitope defined by MAb 1H7.4, 5H4.3, 3D1.4 or 1G5.3 or peptide name identified by MAb 1G5.4-A1-C3 and/or 1C6.3 through their reactions with their ELK/KLE-type or KELK/KLEK-type motifs on the DENV-2 NS1 glycoprotein with their amino acid sequence location (N-TM = peptide N-TERM).
  3. cRelative antibody discriminating specificity (RADS) value, determined from the average ELISA absorbance values of the MAbs or mouse PAb pools against synthetic peptides prepared in the natural/reverse orientations (fold difference of absorbance against the natural orientated peptide sequence/reverse orientated peptide sequence). MAb and PAb RADS values were only determined when they had absorbance (Abs) values of ≥ 0.650 against the peptide prepared in the natural orientation. RADS values of ≥ 1.50-fold or ≥ 2.50-fold are underlined and shown in bold, respectively. Reactions that were Abs < 0.650 against the peptide sequence prepared in the natural orientation are shown by asterixes.