Hepatic reference gene selection in adult and juvenile female Atlantic salmon at normal and elevated temperatures

BMC Research Notes

Table 1 qPCR primers used to amplify fragments of candidate reference genes

Gene	Primer	Sequence (5'→3')	Amplicon size	E*	R²	GenBank #
Hprt1	Hprt1F1	GAT GAT GAG CAG GGA TAT GAC	165 bp	0.963	0.999	BT043501
	Hprt1R1	GCA GAG AGC CAC GAT ATG G
Tbp	TbpF1	TCC CCA ACC TGT GAC GAA CA	117 bp	0.981	0.958	BT059217
	TbpR1	GTC TGT CCT GAG CCC CCT GA
Ef1α	Ef1αF2	GCA CCA CGA GAC CCT GGA AT	94 bp	0.969	0.997	AF321836
	Ef1αR2	CAC GTT GCC ACG ACG GAT AT
β-tubulin	βTubF1	CCG TGC TTG TCG ACT TGG AG	144 bp	0.975	0.998	DQ367888
	βTubR2	CAG CGC CCT CTG TGT AGT GG

ISSN: 1756-0500