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Table 2 Ranking of candidate reference genes

From: Hepatic reference gene selection in adult and juvenile female Atlantic salmon at normal and elevated temperatures

 

Adult experiment

Juvenile experiment

Gene name

BK (corr.)

NF (stab. value)

GN*(M)

BK (corr.)

NF (stab. value)

GN*(M)

Tbp

1 (0.965)

3 (0.106)

1 (0.796)

3 (0.852)

3 (0.154)

3 (0.614)

Ef1α

2 (0.925)

2 (0.09)

3 (0.878)

1 (0.936)

2 (0.146)

1 (0.537)

Hprt1

3 (0.923)

1 (0.081)

1 (0.752)

2 (0.912)

1 (0.065)

1 (0.568)

β-tubulin

4 (0.873)

4 (0.122)

4 (0.913)

-

-

-

  1. BestKeeper (BK), Norm Finder (NF) and GeNorm (GN); (corr.) = correlation between candidate gene and BestKeeper index, (stab. value) = stability value calculated by NormFinder and (M) = stability value calculated by GeNorm with all genes included. GN ranks were determined through step-wise elimination of the least stable gene. For NF and GN a lower value is indicative of a more stable gene. For BK a higher value is indicative of a more stable gene. - β-tubulin was not analysed in the juvenile study. * GeNorm selects the best pair of candidate genes, not a single gene