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Figure 2 | BMC Research Notes

Figure 2

From: Akt and phospholipase Cγ are involved in the regulation of growth and migration of MDA-MB-468 breast cancer and SW480 colon cancer cells when cultured with diabetogenic levels of glucose and insulin

Figure 2

Tumor cell proliferation with high glucose and insulin. MDA-MB-468 breast cancer and SW480 colon cancer cells significantly increased proliferation rates after addition of insulin (100 ng/ml) and doubling glucose concentrations. Even higher proliferative capacity was seen when cells were simultaneously stimulated with glucose and insulin. SW480 cells showed consistently higher proliferation rates than MDA-MB-468 cells. Addition of AKT-inhibitor A6730 and PLCγ-inhibitor U73122 significantly reduced tumor cell proliferation to levels comparable with the control. P-values reflect 5.5 mM glucose plus insulin and 11 mM glucose against control, 11 mM glucose plus insulin against 11 mM glucose, and both culture conditions including the inhibitors against 11 mM glucose plus insulin. Thereby, *p <0.05; **p < 0.001; ***p < 0.0001.

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BMC Research Notes

ISSN: 1756-0500

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