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Figure 3 | BMC Research Notes

Figure 3

From: Akt and phospholipase Cγ are involved in the regulation of growth and migration of MDA-MB-468 breast cancer and SW480 colon cancer cells when cultured with diabetogenic levels of glucose and insulin

Figure 3

Tumor cell migration with high glucose and insulin. MDA-MB-468 breast cancer and SW480 colon cancer cells reduced migratory activity (MA) after addition of Akt-inhibitor A6730 and PLCγ-inhibitor U73122 when compared to cells stimulated with increased glucose and insulin (A). MA is the percentage of cells moving at a particular 15 min interval. Both cell lines significantly increased average migratory activity (AMA, B) and average distance migrated (ADM, C) after stimulation with glucose and insulin. AMA is the average percentage of moving cells, calculated from all 15 min intervals in a 10 h period. ADM refers to the average distance in μm that a tumor cell covered during 10 h of analysis. Akt-inhibitor A6730 and PLCγ-inhibitor U73122 significantly reduced AMA and ADM when compared to cells stimulated with increased glucose and insulin, emphasizing the regulatory functions of Akt and PLCγ when glucose and insulin concentrations are high. P-values reflect 5.5 mM glucose plus insulin and 11 mM glucose against control, 11 mM glucose plus insulin against 11 mM glucose, and both culture conditions including the inhibitors against 11 mM glucose plus insulin. Thereby, *p < 0.05; **p < 0.001; ***p < 0.0001.

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