Figure 3From: Microarray-based uncovering reference genes for quantitative real time PCR in grapevine under abiotic stressOptimal number of reference genes required for effective normalisation. Optimal number of reference genes required for effective normalisation. The pairwise variation (Vn/Vn/n+1) was analysed between the normalisation factors NFn and NFn + 1 using the software GeNorm to determine the optimal number of reference genes required for RT-qPCR data normalisation in three different situations (n and n + 1 as in the ranking of Table 3).Back to article page