Skip to main content

Table 3 Ranking of reference genes using ΔCq and GeNorm

From: Microarray-based uncovering reference genes for quantitative real time PCR in grapevine under abiotic stress

  ΔCq GE Norm
    M values of Ref genes
Ref. Genes Sum of Ref Gene values Ranking Greenhouse Field All samples
act 40 2 1.834 0.503 1.88
L2 137 5 3.604 0.735 3.55
VH1-IK 408 7 1.974 0.496 1.884
aladin-related 1243 8 2.166 0.603 2.072
TIF 200 6 1.921 0.552 1.829
TIF-GTP 110 4 1.89 0.594 1.837
PADCP 39 1 2.092 0.674 2.007
ubiq 54 3 1.885 0.58 1.817
  1. Ranking of reference genes using two different methods, ΔCq and the software GeNorm (http://medgen.ugent.be/~jvdesomp/genorm/). The normalisation with ΔCq was performed with all samples while with GeNorm it was performed with all samples, with greenhouse samples and with field samples individually.