BMC Research Notes

Table 2 Summary of geNorm and Normfinder gene stability values

From: Bone to pick: the importance of evaluating reference genes for RT-qPCR quantification of gene expression in craniosynostosis and bone-related tissues and cells

Mouse suture tissues				Human suture cells				Kusa 4b 10 cells
geNorm		Normfinder		geNorm		Normfinder		geNorm		Normfinder
18 S rRNA	0.95	Eif4a2	0.477	ACTB	0.77	ACTB	0.716	Rpl13a	0.67	Gapdh	0.249
Eif4a2	0.84	18 S rRNA	0.390	EIF4A2	0.67	EIF4A2	0.687	Gapdh	0.62	Rpl13a	0.237
Sdha	0.75	Atp5b	0.340	SDHA	0.63	GAPDH	0.465	B2m	0.56	B2m	0.200
Rpl13a	0.67	Sdha	0.315	B2M	0.60	SDHA	0.461	Sdha	0.50	Sdha	0.184
B2m	0.63	Cyc1	0.289	TOP1	0.57	TOP1	0.422	Ywhaz	0.48	Ywhaz	0.135
Actb	0.59	Ywhaz	0.274	RPL13A	0.53	B2M	0.348	Ubc	0.44	Canx	0.097
Atp5b	0.55	Actb	0.255	SF3A1	0.49	CYC1	0.337	Actb	0.42	Ubc	0.091
Ywhaz	0.52	Gapdh	0.224	YWHAZ	0.41	YWHAZ	0.337	Atp5b	0.39	Actb	0.086
Ubc	0.49	Rpl13a	0.194	CYC1	0.36	ATP5B	0.295	Canx*	0.35	Atp5b	0.074
Canx	0.47	B2m	0.188	GAPDH	0.31	18 S rRNA	0.262	18 s rRNA*	0.35	18 S rRNA	0.048
Cyc1*	0.34	Ubc	0.141	18 S rRNA*	0.28	RPL13A	0.217
Gapdh*	0.34	Canx	0.081	ATP5B*	0.28	SF3A1	0.202

Genes are ranked from the least stable (top) to the most stable (bottom). Stability values are shown for geNorm and Normfinder and lower numbers indicate greater stability. Asterisk indicates equal ranking. The optimal number of reference genes to use, based on their V values (geNorm) or intergroup/intragroup variations (Normfinder) are marked as bold.

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ISSN: 1756-0500

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