Utilizing the slot blot assay to detect the overall DNA methylation level of newborn piglets. (a) Increasing amount of porcine genomic DNA (a mix of all the samples detected) ranging from 0.5 to 7 μg (marked underneath each band) was blotted onto the membrane. (b) The observed band intensity was plotted against the amount of DNA blotted. (c) The logarithm of band intensity was plotted against the amount of DNA blotted. (d) 4 μg of genomic DNA from three types of tissues of male Large White and Erhualian piglets was used (n = 6). Data are presented as means ± SE. * means significant difference between pig breed (p < 0.05).