Figure 1

Effects of DMSO and formamide on the PCR amplification of GC-rich templates. Typical examples of the effects of DMSO and formamide addition at different concentrations on the PCR amplification of 2–3 kb GC-rich DNA templates. A) Effect of DMSO on PCR amplification of che1P (top) and tlp2 (bottom) as observed by gel electrophoresis of total PCR products. The gels were stained with ethidium bromide and photographed. B) Effect of formamide on PCR amplification of che1P (top) and tlp2 (bottom) in comparable experiments.