Experimental measurements and model construction. Wild-type S. cerevisiae (A, B) and C. albicans (C-D) cells were exposed to hyperosmotic condtions. (A) S. cerevisiae: 0.2 and 0.5 M NaCl; (B) S. cerevisiae: 1 M NaCl; (C) C. albicans: 0.5 and 1 M NaCl. (D) C. albicans: 1 M NaCl. Hog1 activity, intracellular glycerol and total glycerol were measured in time courses of up to 1 hour. Experimental data for S. cerevisiae in A and B are published results . C. albicans data are from this study. Model predictions were plotted separately from experimental measurements.