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Table 1 DNA extraction results

From: Extraction of DNA from plant and fungus tissues in situ

Tissues Yield (ng/mg)
Species Fresha,b 48 houra,b,c 72 houra,b,d
Fungus basidiocarps    
 Agaricus bispora 2.0 (−) 4.5 (+/−) nd
 Pleurotus ostreatus 1.0 (−) 0.5 (+) nd
Plant embryonic axes    
 Cucurbita maxima Y (−) Y (+/−) nd
 Picea pungens Y (−) Y (+/−) nd
 Pinus sylvestris Y (−) Y (+/−) nd
 Pisum sativum Y (−) Y (+/−) nd
 Triticum aestivum 218 (−) 203 (+/−) nd
 Vicia faba 92 (−) 82 (+) nd
 Zea mays 25 (−) 23 (+) nd
Plant embryonic axes with cotyledons
 Citrullus lantus Y (−) Y (+/−) nd
 Cucumis melo Y (−) Y (+/−) nd
 Cucurbita maxima 0 0 nd
 Cucurbita pepo Y (−) Y (+/−) nd
Plant leaves    
 Adiantum capillis-veneris 2.8 (−) nd 0
 Araucaria heterophylla 6.6 (−) nd 2.0 (+/−)
 Chamaedora elegans 11.1 (−) nd 2.8 (+/−)
 Cordyline fructicosa 3.1 (−) nd 2.8 (+)
 Cyperus papyrus 0.9 (−) nd 3.9 (+)
 Davallia fejeensis 2.2 (−) nd 7.5 (+/−)
 Fittonia verschaffeltii 8.8 (−) nd 6.8 (+/−)
 Guzmania lingulata 5.2 (−) nd 0
 Jasmium sambac 3.6 (−) nd 2.3 (+)
 Nemathanthus gregarius 0.5 (−) nd 0.3 (+)
 Picea pungens Y (−) Y (+/−) nd
 Pilea nummularifolia 0.4 (−) nd 0
 Pinus sylvestris Y (−) Y (+/−) nd
 Populus canadensis Y (−) Y (+/−) nd
 Saintpaulia ionantha 0.3 (−) nd 0.5 (+)
Plant seeds    
 Capsicum annuum Y (−) Y (+/−) nd
 Daucus carrota Y (−) Y (+/−) nd
 Solanum lycopersicum Y (−) Y (+/−) nd
  1. aSamples were dissected, followed by immediate DNA extraction or freezing at −20°C (see text) and then subjected to DNA extraction at a later time. The tissue samples were from 25 mg to 750 mg. DNA amounts were estimated from ethidium bromide fluorescence compared to standards on the same gel. Yields were calculated based on aliquots from the total extracted DNA. The letter “Y” indicates successful extraction where yields were not quantified.
  2. bDegree of degradation was based on the sharpness of the high molecular weight band and diffuse fluorescence at lower molecular weights. A plus (+) indicates prominent degradation, a plus/minus (+/−) indicates some degradation, and a minus (−) indicates little or no degradation was evident.
  3. cSamples were left out on a laboratory bench top for 48 hours, followed by DNA extraction (plant leaves), or they were frozen at −20°C for DNA extraction at a later time. Not determined = nd.
  4. dSamples were left out on a laboratory bench top for 72 hours, followed by DNA extraction. Not determined = nd.