Subcellular distribution of various Rab family small GTP binding proteins. (A) Example images showing mCherry-Rab proteins expressed in HeLa cells. (B) Example dual colour images of mCherry-Rab proteins co-immunostained with the cis-Golgi marker GM130. (C) Box plot showing rank-weighted co-localization coefficients of the various mCherry-Rab proteins with GM130. The central lines within each plot represent the median co-localization values. Statistical analysis (ANOVA) revealed overall significant difference between the 6 Rabs (p < 0.001).