DSCR9 gene simultaneous expression in placental, testicular and renal tissues from baboon (papio hamadryas)

BMC Research Notes

Table 1 Primers and PCR conditions

Oligo name	Oligo sequence	Orientation	Primer set	Substrate to PCR	Initial denatur alization	Amplification program			Cycles	Final elongation	Amplicon size
Oligo name	Oligo sequence	Orientation	Primer set	Substrate to PCR	Initial denatur alization	Denaturalization	Alignment	Elongation	Cycles	Final elongation	Amplicon size
OLIGO-rnaF	CTTGGCGCTAAGCTGCCGC	Forware	AMPLICON -mRNA	mRNA	94°/3.5 min	94°/30 seg	60°/45 seg	72°/30 seg	42	72°/15 min	726 pb
OLIGO-rnaR	CCTGCTCTGGAGTCTTGGTG	Reverse
OLIGO-gene1F	AGCTGGCACTCCCCAGAAT	Forware	OLIGO-gene1	gDNA	94°/5 min	94°/1 min	60°/45 seg	72°/1 min	35	72°/10 min	946 pb
OLIGO-gene1R	GGCTGAGGCACAGAGAAACT	Reverse
OLIGO-gene2F	CTCCCTACCAAAGTGGCTAG	Forware	OLIGO-gene2	gDNA	94°/5 min	94°/1 min	60°/45 seg	72°/35 seg	30	72°/10 min	769 pb
OLIGO-gene2R	TGTGGAAAGTTGGGGTTTTC	Reverse
OLIGO-gene3F	GAAAACCCCAACTTTCCACA	Forware	OLIGO-gene3	gDNA	94°/5 min	94°/1 min	60°/45 seg	72°/30 seg	30	72°/10 min	723 pb
OLIGO-gene3R	CCAGGCGAGCAGTCTGTAAC	Reverse
OLIGO-gene4F	CCTCTCCTGCAACCAATCAG	Forware	OLIGO-gene4	gDNA	94°/5 min	94°/1 min	60°/45 seg	72°/45 seg	30	72°/10 min	807 pb
OLIGO-gene4R	CCCGAATATCCTGGGCTCT	Reverse
OLIGO-gene5F	CCGGAAGAGCCCAGGATA	Forware	OLIGO-gene5	94°/5 min	94°/1 min	60°/45 seg	72°/1 min	gDNA	35	72°/10 min	967 pb
OLIGO-gene5R	CCGTTTTGGCAGGAATACAT	Reverse

ISSN: 1756-0500