Sub-fragment number
|
Start*
|
End*
|
Length
|
5′-flanking RE
|
3′-flanking RE
|
Clones sequenced**
|
Clones with the correct sequence, %
|
---|
1
|
8532
|
9117
|
586
|
(Xho
I
)
|
Avr II
|
3
|
100
|
2
|
9114
|
9800
|
687
|
Xba I
|
Bam HI
|
3
|
67
|
3
|
9795
|
10573
|
779
|
Bam HI
|
Avr II
|
3
|
67
|
4
|
10570
|
11208
|
639
|
Spe I
|
Xho I
|
3
|
100
|
5
|
11205
|
12491
|
1287
|
Sal I
|
(Nco I)
|
4
|
100
|
- Nucleotide positions are numbered according to the GenBank entry AY188393. Restriction sites that were used for sub-cloning the assembled fragments into the expression vectors are bracketed.
- *Start and end positions include the corresponding restriction endonuclease recognition sites.
- **Following fragment assembly, clones were re-sequenced using specific primers; no mutations were found.
- RE restriction endonuclease.