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Table 1 Sub-fragments of the Chinese hamster elongation factor 1 alpha upstream flanking region

From: A modular assembly cloning technique (aided by the BIOF software tool) for seamless and error-free assembly of long DNA fragments

Sub-fragment number Start* End* Length 5′-flanking RE 3′-flanking RE Clones sequenced** Clones with the correct sequence, %
1 8532 9117 586 (Xho I ) Avr II 3 100
2 9114 9800 687 Xba I Bam HI 3 67
3 9795 10573 779 Bam HI Avr II 3 67
4 10570 11208 639 Spe I Xho I 3 100
5 11205 12491 1287 Sal I (Nco I) 4 100
  1. Nucleotide positions are numbered according to the GenBank entry AY188393. Restriction sites that were used for sub-cloning the assembled fragments into the expression vectors are bracketed.
  2. *Start and end positions include the corresponding restriction endonuclease recognition sites.
  3. **Following fragment assembly, clones were re-sequenced using specific primers; no mutations were found.
  4. RE restriction endonuclease.