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Table 4 Differences between the Chinese hamster elongation factor 1 alpha downstream flanking fragments obtained in this study and published data

From: A modular assembly cloning technique (aided by the BIOF software tool) for seamless and error-free assembly of long DNA fragments

# Sub-fragment number Position AFTD01093963.1 Present work AY188393
1 1 2349-2353 GTCCC ATATT ATATT
2 1 2378 G C C
3 1 2510 C A A
4 1 2555 A C C
5 1 2559 T A A
6 1 2587/2588 - A A
7 1 2842 C C -
8 2 3305 G G -
9 2 3674 G G C
10 3 3893 T T G
11 5 5372 A G A
12 6 6160 G G -
  1. Nucleotide changes were observed in only one of the three aligned sequences (shown underlined). Nucleotide positions are shown according to the GenBank entry AFTD01093963.1.