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Table 1 - Nucleotide Sequences of Oligonucleotides used for PCR Amplification

From: URPD: a specific product primer design tool

Species ID

Primers

Nucleotide sequence (5’-3’)

Primer length (bps)

Tm (°C)

GC%

PCR targets

Size (bp)

ATCC 19606

bla OXA212F

GTGCTTCGACCGAGTATG

18

45.68

55.56

blaOXA resistance gene

496

 

bla OXA707R

ACAACCATCCAGTTAACC

18

41.12

44.44

  

ATCC 19606

bla OXA274F

GAGCACCATAAGGCAACC

18

45.68

55.56

blaOXA resistance gene

451

 

bla OXA724R

TATTCCCTTGAGGCTGAAC

19

44.32

47.37

  

ATCC 6538p

pbp 514F

ATGCTTTACGACAAAGTTTC

20

41.25

35.00

penicillin-binding protein

330

 

pbp 843R

TCTCAGCTAACATGTATGC

19

42.17

42.11

  
  1. The primers designed using accession number HQ425495.1 and AF540028.1 for blaOXA and pbp, respectively. F and R under the primers field represent forward primer and reverse primer, respectively. The number under the primers field represents the exact placement of the primer within the target sequence, e.g., the 212 F represents that the forward primer has the exact placement of 212 within the target sequence.