Figure 2

– Percentage of infected ears yielding a middle ear effusion and bacterial titers in middle ear effusions. A) Percentage of successfully tapped ears infected with either the wildtype strain 86-028NP (black columns) or the tonB mutant strain HI2280 (grey columns) yielding a middle ear effusion. Using Fisher’s Exact test to compare percentages of ears infected with either strain yielding effusions on given days: on day 4 P = 0.0325, on day 7 P = 0.0007, on day 10 P = 0.0004, on day 14 P = 0.0256 and on day 17 P = 0.015. B) Bacterial titers in middle ear effusions from ears infected with the wildtype strain 86-028NP (black columns). No bacteria were detected in middle ear effusions recovered on day 4 from ears infected with the tonB mutant strain HI2280. On days 7 through 17 no middle ear effusions were recovered from ears infected with the tonB mutant strain HI2280.