Figure 1
From: Construction and use of a prokaryotic expression system for Helicobacter pylori AhpC
Schematic diagram of cloning and transformation of tsaA gene. Whole tsaA gene having BamH1 and EcoR1 sites was ligated with pGEX-6p-2 that had been cut with the same enzymes to generate pGEX-tsaA. This plasmid was used to transform E. coli, which produces rGST-AhpC fusion protein under induction with IPTG.