Figure 3
From: Construction and use of a prokaryotic expression system for Helicobacter pylori AhpC
A. Growth curves showing OD 600 of E. coli BL21 cells for induction of AhpC with 0.1 and 1 mM IPTG at 0, 2 and 4 hours of growth. 1A and 2A are duplicate samples. B. SDS-PAGE showing ~52 kDa target recombinant fusion protein (rGST-AhpC). Lane 1: Kaleidoscope colour marker (BioRad); Lanes 2–4 Protein samples induced with 0.1 mM IPTG at 0, 2 and 4 hours respectively; Lanes 6–7: Protein samples induced with 1 mM IPTG; Lanes 5 & 8: Blank controls.