Table 1 Strains and plasmid used in the study
From: Construction and use of a prokaryotic expression system for Helicobacter pylori AhpC
Strain | Genotype/description | Source |
|---|---|---|
E. coli | ||
DH10B | Cloning strain | Promega |
BL21 (DE3) | E. coli B F– dcm ompT hsdS (rB–mB–) gal λ(DE3) [pLysS Camr] | Promega |
H. pylori | ||
G27 | Wild type | Nina R. Salama, University of Washington, Seattle, WA |
26695 | Wild type | Karen M. Ottemann, UC Santa Cruz, CA |
Plasmids | ||
pGEX-6p-2 | GST fusion plasmid | Novagen |