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Table 1 Bacterial strains and plasmids used in this study

From: ToxR of Vibrio cholerae affects biofilm, rugosity and survival with Acanthamoeba castellanii

Strain or plasmid Description Reference
V. cholerae O1 E1Tor Inaba Strain A1552, Wild type [13]
V. cholerae ΔtoxR Transcriptional activator deletion mutant of Strain A1552 This study
E. coli DH5α Φ80dlac ZΔM15 recA1gyrA96 thi-1 hsdR 17 (rk- mk+) supE44 [14]
Sm10λpir relA1deoR Δ(lacZYA-argF)U169 [15]
  thi-1, thr, leu, tonA, lacY, supE,  
Plasmid Puc 18 ApR lacZaovi colE1 [16]
Plasmid PCVD442 oriR6K mobRP4 sacB Apr this study