Table 1 Bacterial strains and plasmids used in this study
From: ToxR of Vibrio cholerae affects biofilm, rugosity and survival with Acanthamoeba castellanii
Strain or plasmid | Description | Reference |
|---|---|---|
V. cholerae O1 E1Tor Inaba | Strain A1552, Wild type | [13] |
V. cholerae ΔtoxR | Transcriptional activator deletion mutant of Strain A1552 | This study |
E. coli DH5α | Φ80dlac ZΔM15 recA1gyrA96 thi-1 hsdR 17 (rk- mk+) supE44 | [14] |
Sm10λpir | relA1deoR Δ(lacZYA-argF)U169 | [15] |
thi-1, thr, leu, tonA, lacY, supE, | ||
recA::Rp4-2-Tc::Mu,KmR,(λpir) | ||
Plasmid Puc 18 | ApR lacZaovi colE1 | [16] |
Plasmid PCVD442 | oriR6K mobRP4 sacB Apr | this study |