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Table 1 The sequences of primers selected to perform qRT- PCR analysis

From: Unmasking epithelial-mesenchymal transition in a breast cancer primary culture: a study report

Gene Name GenBank Forward primer Reverse primer
ACTA2 (α-SMA) NM_001141945.1 5′-gctgttttcccatccattgtg-3′ 5′-ttggtgatgatgccatgttct-3′
CDH1 (E-cadherin) NM_004360.3 5′-ccaagtgcctgcttttgatga-3′ 5′-cccctacccctcaactaac-3′
CDH2 (N-cadherin) NM_001792.3 5′-gacaatgcccctcaagtgtt-3′ 5′-ccattaagccgagtgatggt-3′
FN1(Fibronectin 1) NM_212482.1 5′-ggaaagcatatgcagccaac-3′ 5′-ctacagtattgcgggccaga-3′
KRT5 (CK5) NM_000424.3 5′-tttgtctccaccacctcctc-3′ 5′-cctgggaaccaaagaatgtg-3′
KRT6 (CK6) NM_005554.3 5′-caagctcaccttccaggact-3′ 5′-gagtgtgagaggctggagga-3′
KRT8 (CK8) NM_002273.3 5′-gggaagctggtgtctgagtc-3′ 5′-ctcctgttcccagtgctacc-3′
KRT18 (CK18) NM_000224.2 5′-ccagtctgtggagaacgaca-3′ 5′-atctgggcttgtaggccttt-3′
RN18S1(rRNA18S) NR_003286.2 5′-aaacggctaccacatccaag-3′ 5′-caattacagggcctcgaaag-3′
SNAI1 (Snail) NM_005985.3 5′-gcgagctgcaggactctaat-3′ 5′-ggacagagtcccagatgagc-3′
TGFβ1 NM_000660.4 5′-ccctggacaccaactattgc-3′ 5′-aggcagaagttggcatggta-3′
Twist1 NM_000474.3 5′-aaactggcctgcaaaaccatag-3′ 5′-tgcattttaccatgggtcctg-3′
VIM (vimentin) NM_003380 5′-aacaaccgacactcctacaaga-3′ 5′-tggttggatacttgctggaaa-3′
ZEB1 NM_001128128.2 5′-gaaaaaccacaaggggatgag-3′ 5′-gcttgactttcagccctgtc-3′
ZEB2 NM_014795.3 5′-atggggccagaagccacgat-3′ 5′-gtcgactgcatgaccatcgc-3′