Figure 6
From: Characterisation of a natural variant of the γ-butyrolactone signalling receptor
γ-Butyrolactone production in liquid S. coelicolor cultures using the kanamycin bioassay. The kanamycin bioassay [21] was used to detect γ-butyrolactone production in S. coelicolor in liquid SMM at four time points (tp 1–4) during different phases of growth indicated with eT, mT, lT and S (e arly, m id, l ate t ransition, and s tationary phase; also see Table 2, GC 2). Growth of a bioassay indicator strain on kanamycin supplemented medium is shown. Kanamycin resistance and thus growth is induced by the presence of γ-butyrolactones. Extracts from strains LW34 and LW33, containing ScbRM145 and ScbRM600, respectively, resembled each other in the induction of growth, however, with LW34 sparse growth seems to be induced already at time point 2 and the area of growth is increased compared to LW33 with the late transition phase sample. Chemically synthesised S. coelicolor γ-butyrolactone (SCB1) was used as positive, the solvent methanol as negative control.