Figure 7
From: Characterisation of a natural variant of the γ-butyrolactone signalling receptor
Transcriptional analysis of redD , actII -orf4, cpkO , scbA and scbA and scbR M145/M600 using qRT-PCR. qRT-PCR analysis of the transcription of redD, actII-orf4, cpkO, scbA and scbRM145/M600 using cDNA synthesized from RNA isolated from liquid SMM cultures of S. coelicolor LW34 (scbRM145) and LW33 (scbRM600). Samples were taken at four time points (tp 1–4) during different phases of growth indicated with eT, mT, lT and S (e arly, m id, l ate t ransition, Sand s tationary phase). Gene expression is shown as fold-change relative to the LW34 time point 1 early transition phase sample. Error bars indicate the standard deviation of each three technical replicates. For numerical data see Additional file 4A and 4B.