High prevalence of multidrug resistance in bacterial uropathogens from Kathmandu, Nepal

BMC Research Notes

Table 4 Conjugational transfer of multidrug resistance phenotypes in E. coli

Donor	Transconjugant						^cMIC (μg/ml) of antibiotics towards donors and transconjugants							^aConjugation frequency
Resistance pattern	Plasmid (kb)	^bESBL activity	Resistance pattern	Plasmid (kb)	^bESBL activity		Am	C	Cf	Cfx	G	Nx	Co
Am, Cf, Ce, Cfx, Co, Of, Nx, Ca, C	32.5	-	Am, Cf, Cfx, Co, Of, Nx	32.5	-	D	> 512		> 256	> 512		128	512	0.3 × 10^-7
						T	> 512		64	> 512		64	512
Am, Cf, Ce, Cfx, Co, Of, Nx, Ca, C, G, Ci	32.5	+	Am, Cf, Cfx, Co, Of, Nx	32.5	+	D	> 512		> 256	> 512		> 256	> 512	0.6 × 10^-7
						T	> 512		64	512		64	> 512
Am, Cf, Co, Nx, Na, Of, Ca, Ci	32.5	+	Am, Cf, Cfx, Co, Of, Nx	32.5	+	D	> 512		> 256	> 512		> 256	> 512	1.5 × 10^-7
						T	> 512		128	> 512		64	512
Am, Cf, Ce, Cfx, Co, Of, Nx, Ca, G, Ci	32.5	-	Am, Cf, Cfx, Co, Of, Nx	32.5	-	D	512		> 256	> 512		> 256	> 512	0.6 × 10^-7
						T	512		64	512		64	512
Am, Cf, Ce, Cfx, Co, Of, Nx, Ca, C, G, Ci	32.5, 4.7	+	Am, Cf, Cfx, Co, Of, Nx, C, G	32.5	+	D	> 512	512	> 256	> 512	> 64	> 256	> 512	1.2 × 10^-7
						T	> 512	512	128	512	> 64	128	> 512
Am, Cf, Ce, Cfx, Co, Of, Nx, Ca, G, Ci	51	+	Am, Cf, Cfx, Co, Of, Nx	51	-	D	512		> 128	512		128	512	0.5 × 10^-7
						T	256		32	256		32	512
Am, Cf, Ce, Cfx, Co, Of, Nx, Ak, Ca, G, Ci	38	+	Am, Cf, Cfx, Co, Of, Nx	38	+	D	> 512		> 256	> 512		> 256	> 512	0.8 × 10^-7
						T	> 512		64	> 512		64	> 512
Am, Cf, Co, Nx, Ca, Ci	38	-	Am, Cf, Cfx, Co, Of, Nx	38	-	D	512		128	> 512		128	512	0.5 × 10^-7
						T	256		64	256		64	512
Am, Cf, Ce, Cfx, Co, Of, Nx, C, Ca, G, Ci	32.5, 6.5	+	Am, Cf, Cfx, Co, Of, Nx	32.5, 6.5	+	D	512		128	> 512		128	> 512	0.3 × 10^-7
						T	512		64	> 512		64	> 512
Am, Cf, Ce, Cfx, Co, Of, Nx, Ca, Ci	32.5	+	Am, Cf, Cfx, Co, Of, Nx	32.5	+	D	> 512		128	> 512		128	> 512	1.2 × 10^-7
						T	> 512		64	> 512		64	512

^aConjugation frequency is the number of transconjugants per donor cell
^bESBL = extended-spectrum β-lactamase
^cMIC = minimum inhibitory concentration of antibiotic at which visible growth of isolates were inhibited
Am-amoxycillin, Ce-cefotaxime, Cfx-cefixime, Cf-ciprofloxacin, Co-cotrimoxazole, Nf-nitrofurantoin, Nx-norfloxacin, Of-ofloxacin, Ak-amikacin, C-chloramphenicol, Ca-ceftazidime, Ci-ceftriaxone, G-gentamycin, D-donor, T-transconjugant

ISSN: 1756-0500