Figure 1
From: Biogenesis of protein bodies during vicilin accumulation in Medicago truncatula immature seeds
Embryo cell micrographs in seed sections at early-mid maturation. (A-D) Light microscopy detection and characterisation of protein bodies in seed sections. (A) The initiation of vacuole fragmentation is shown 16 DAP, storage bodies are pink due to the metachromatic Toluidine blue (TBO) staining. (B) The protein bodies (pb), revealed with Naphtol blue black protein staining, are more abundant at 20 DAP. (C-D) Illustrate the pattern of the vacuolar compartements (vc) at 20 DAP, with the fast green protein staining (C). They correspond to black holes (D) indicated by (*) using the fluorescent propidium iodide. (E) The mitotic figures observed in the abaxial region are indicated by arrows. (F-G) Electron micrographs illustrate the abundance of plastids (pl) and starch granules (s) in (F), the presence of plasmodesmata in the cell wall (cw) is shown in (G). The scale bar corresponds to 4 μm in A), (B); 8 μm in (C), (D); 7 μm in (E); 1,5 μm in F; 0,4 μm in G.